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talon resin  (TaKaRa)


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    Structured Review

    TaKaRa talon resin
    Talon Resin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1953 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/talon+metal+affinity+resin/TALON+Metal+Affinity+Resin/pmc13089183-296-6-8
    Average 99 stars, based on 1953 article reviews
    talon resin - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Clarification Assay:

    Article Title: Geissoschizine scaffolding enzymes shape monoterpene indole alkaloid biosynthesis
    Article Snippet: .. After clarification, the supernatant was incubated with TALON Metal Affinity Resin (Takara), pre-equilibrated with NPI-10 buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 10 mM imidazole, pH 8), for 2 h at 4°C under gentle agitation. ..

    Incubation:

    Article Title: Geissoschizine scaffolding enzymes shape monoterpene indole alkaloid biosynthesis
    Article Snippet: .. After clarification, the supernatant was incubated with TALON Metal Affinity Resin (Takara), pre-equilibrated with NPI-10 buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 10 mM imidazole, pH 8), for 2 h at 4°C under gentle agitation. ..

    Article Title: pH-mediated activation of the lysosomal arginine sensor SLC38A9.
    Article Snippet: To purify SLC38A9 protein and its variants, the membrane fraction was thawed and solubilized with 2% ndodecyl-β-D-maltopyranoside (DDM; Anatrace, Maumee, OH, USA), 0.2% Cholesteryl Hemisuccinate Tris salt (CHS; Anatrace) in 20 mM Tris pH 8.0, and 150mM NaCl with 10% glycerol for 2 h at 4 °C. .. Following another ultra-centrifugation at 130 000× g for 45 min, the supernatant was loaded onto TALON Metal Affinity Resin (Clontech, San Jose, CA, USA) and incubated at 4 °C overnight. ..

    Article Title: Elucidating the molecular interplay between LRRK2 and Rab GTPases
    Article Snippet: .. The cleared lysate was incubated with TALON Metal Affinity Resin (Takara), followed by washing with lysis buffer containing 10 mM imidazole. ..

    Article Title: NIMA-related kinases redundantly regulate vegetative and reproductive development in Arabidopsis thaliana.
    Article Snippet: Recombinant GST-NEK proteins were expressed in the presence of 1 mM isopropyl 1-thio-beta-galactopyranoside at 16°C for 12 hr and purified with glutathionesepharose 4B (GE Healthcare) according to the manufacturers’ instruction. .. Recombinant 6xHis-tagged tubulins and maltose-binding-protein (MBP)-tagged proteins were induced by the addition of 0.2 mM isopropyl 1-thio-beta-galactopyranoside and 0.1% rhamnose and further incubated at 20°C for 16 h. The Histagged tubulins were purified with TALON metal affinity resin (Clontech) according to the manufacturers’ protocol. .. MBP-tagged FT/TFL1 were purified with maltose resin (NEB) according to the manufacturers’ method.

    Gentle:

    Article Title: Geissoschizine scaffolding enzymes shape monoterpene indole alkaloid biosynthesis
    Article Snippet: .. After clarification, the supernatant was incubated with TALON Metal Affinity Resin (Takara), pre-equilibrated with NPI-10 buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 10 mM imidazole, pH 8), for 2 h at 4°C under gentle agitation. ..

    Purification:

    Article Title: Green genetically encoded IP 3 biosensor for hierarchical analysis of its signaling network
    Article Snippet: The pellets were resuspended in phosphate-buffered saline at pH 7.4 containing 0.5% (v/v) Triton X-100 (PBS-Triton), and the cells were disrupted using a high-pressure Cell Disruptor (Constant Systems Ltd., Daventry, UK). .. The lysate was centrifuged, and the supernatant was subjected to purification using TALON Metal Affinity Resin (TaKaRa Bio, Shiga, Japan). ..

    Article Title: NIMA-related kinases redundantly regulate vegetative and reproductive development in Arabidopsis thaliana.
    Article Snippet: Recombinant GST-NEK proteins were expressed in the presence of 1 mM isopropyl 1-thio-beta-galactopyranoside at 16°C for 12 hr and purified with glutathionesepharose 4B (GE Healthcare) according to the manufacturers’ instruction. .. Recombinant 6xHis-tagged tubulins and maltose-binding-protein (MBP)-tagged proteins were induced by the addition of 0.2 mM isopropyl 1-thio-beta-galactopyranoside and 0.1% rhamnose and further incubated at 20°C for 16 h. The Histagged tubulins were purified with TALON metal affinity resin (Clontech) according to the manufacturers’ protocol. .. MBP-tagged FT/TFL1 were purified with maltose resin (NEB) according to the manufacturers’ method.

    Lysis:

    Article Title: Elucidating the molecular interplay between LRRK2 and Rab GTPases
    Article Snippet: .. The cleared lysate was incubated with TALON Metal Affinity Resin (Takara), followed by washing with lysis buffer containing 10 mM imidazole. ..

    Protein Purification:

    Article Title: A phage repressor involved in the DNA-damage response of Francisella
    Article Snippet: .. Supernatant was recovered and then used for protein purification through Talon Metal Affinity resin (Clontech), as previously described ( ). ..

    Recombinant:

    Article Title: NIMA-related kinases redundantly regulate vegetative and reproductive development in Arabidopsis thaliana.
    Article Snippet: Recombinant GST-NEK proteins were expressed in the presence of 1 mM isopropyl 1-thio-beta-galactopyranoside at 16°C for 12 hr and purified with glutathionesepharose 4B (GE Healthcare) according to the manufacturers’ instruction. .. Recombinant 6xHis-tagged tubulins and maltose-binding-protein (MBP)-tagged proteins were induced by the addition of 0.2 mM isopropyl 1-thio-beta-galactopyranoside and 0.1% rhamnose and further incubated at 20°C for 16 h. The Histagged tubulins were purified with TALON metal affinity resin (Clontech) according to the manufacturers’ protocol. .. MBP-tagged FT/TFL1 were purified with maltose resin (NEB) according to the manufacturers’ method.



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